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Cosumables
Consumables are essential items used during chromatographic analysis, including vials, caps, septa, syringe filters, and solvent bottles. They need to be regularly replaced for proper functioning and to maintain the integrity of the analysis.
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Vials and Caps |
Syringe Filters | Solvent Bottles |
Solid Phase Extraction |
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Vials are available in different materials like glass and polypropylene. Glass vials are inert and do not interact with samples. Amber glass vials protect samples from light. Screw thread vials allow for leak-proof sealing. Crimp top vials use aluminum seals for volatility retention. Caps have ptfe/silicone septa. |
Syringe filters use membranes like PTFE, nylon, mixed cellulose esters for clarity and low binding. Pore sizes like 0.2 μm and 0.45 μm help remove particulates. They filter mobile phase solvents before use. Filters certified for HPLC solvents prevent contamination. | Solvents are stored in amber bottles that prevent light exposure. Bottles have polypropylene screw caps with ptfe liners for leak-proof sealing. Solvent bottles are labeled with the solvent name, date, initials and have batch traceability. Glass bottles necessitate Teflon taps to avoid leakages. |
The purpose of solid-phase extraction (SPE) is to clean the sample before injection. In SPE, you load the sample onto the cartridge (selecting media that retains the analytes of interest) and wash off the impurities. Then, elute analytes of interest for downstream analysis. |
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Tubing and Fittings |
LC Solvents |
GPC clean up |
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To use an HPLC system, plumbing your system with the appropriate tubing and fittings is important, as it impacts your instrument’s up-time. A fitting refers to a system comprised of a nut and ferrule. Determining what fitting system to use depends on the threads of the receiving port, the geometry of the receiving port, size and type of tube used, material of the port, and the amount of pressure expected. |
Solvents are liquids used to extract and or dissolve substances and are the mobile phase in liquid chromatography. For the selection of mobile phase solvents in reverse phase chromatography, HPLC grade or better solvents are always recommended. Whereas LC/MS grade solvents or better are always recommended for UHPLC and mass spectrometry. |
Column clean-up for GPC using acetone/cyclohexane or ethyl acetate/cyclohexane has been developed as an alternative to using dichloromethane to reduce environmental impact. The CLNpak EV-2000 series is specifically designed for GPC clean-up with non-halogenated solvents such as Fractionation Standards (EV-2000). |