Reversed Phase Chromatography (RP)
- Separation is based on the partition equilibrium between stationary phase and mobile phase.
- The polarity of the stationary phase is lower than that of the mobile phase.
- Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
- Use of lower polarity mobile phases fasten the elution.