Reversed Phase Chromatography (RP)

  • Separation is based on the partition equilibrium between stationary phase and mobile phase.
  • The polarity of the stationary phase is lower than that of the mobile phase.
  • Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
  • Use of lower polarity mobile phases fasten the elution.


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