Reversed Phase Chromatography (RP)

• Separation is based on the partition equilibrium between stationary phase and mobile phase.
• The polarity of the stationary phase is lower than that of the mobile phase.
• Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
• Use of lower polarity mobile phases fasten the elution.