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  11. Hydrophilic Interaction Chromatography (HILIC)

Hydrophilic Interaction Chromatography (HILIC)

  • Separation is based on hydrophilic interaction.
  • A high polarity stationary phase is used.
  • Typically, the mobile phase contains a mixture of organic solvents such as acetonitrile and aqueous solvent (water or buffer).
  • Using the higher polarity mobile phase causes a faster elution.
  • Application for the analysis of high polar substance.
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Hydrophilic Interaction Chromatography (HILIC)

Features
Specifications
Related products

Features

VC-50 2D
  1. Modified carboxyl group is suitable for cationic substance analysis including amines.
  2. The dominant separation mode is RP or IEX rather than HICL mode.
NH2P series
NH2P-50
  1. Suitable for saccharide analysis using HILIC mode.
  2. Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period.
  3. Easily regenerated by washing in an alkaline solution.
  4. Appropriate for evaporative light scattering detector, corona charged aerosol detector, and LC/MS
  5. Fulfills USP-NF L82 requirements.
NH2P-40 Provide higher theoretical plate number than NH2P-50 series
VG-50 series
  1. Suitable for saccharide analysis using HILIC mode.
  2. Recovers reducing saccharides with high ratio.
  3. Polymer-based packing material provides excellent. chemical stability and minimum deterioration over an extended time period.
  4. Easily regenerated by washing in an alkaline solution.
  5. Appropriate for evaporative light scattering detector, corona charged aerosol detector, and LC/MS 
VN-50 series
  1. The modified carboxyl group is suitable for cationic substance analysis including amines.
  2. Suitable for oligonucleotide and oligosaccharide separation which is not possible by SEC or conventional HILIC columns.
SC1011-7F, MN-431
  1. Pharmacopoeia method relevant columns.
  2. modified ligand exchange chromatography column.
  3. Only water is required for the analysis of neutral sugars.
  4. Fulfills USP-NF L19 and L22 requirements.
SC1211, DC-613, SZ5532
  1. Separation elements by combination of ligand exchange and HILIC modes.
  2. DC-613 can analyze sugars without removing sodium salt in the sample.
  3. SZ5532 is recommended for the separation of disaccharides or trisaccharides.
  4. SC1211 is suitable for separating sugar alcohols.
  5. DC-613 fulfills USP-NF L22 and L58 requirements.
  6. SZ5532 fulfills USP-NF L22 requirements.
  7. SC1211 fulfills USP-NF L19 and L22 requirements.
KS-801 (), KS-802 (),

SP0810, SC1011
  1. Separates saccharides by combination of ligand exchange and size exclusion modes.
  2. Three types of counter ions are available: ,  and .
  3. Only water is required for the analysis of neutral sugars.
  4. SC1011 fulfills USP-NF L19 and L22 requirements.
  5. SP0810 fulfills USP-NF L22 and L34 requirements.
  6. KS-801 and KS-802 fulfills USP-NF L22 and L58 requirements.
KS-803, KS-804
  1. Suitable for separation of polysaccharides by size exclusion modes.
  2. Can be used in combination with other columns e.g., KS-801 and/ or KS-802
  3. Only water is required for the analysis of neutral sugars.
  4. Fulfills USP-NF L22 and L58 requirements.
VT-50 2D
  1. Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode.
  2. Use of some eluents add ion exchange mode.
  3. Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period
  4. Suitable LC/MS analysis.

Specifications

Functional group Particle size (µm) Base material Column size (mm)
HILIC amino 4 Polyvinyl alcohol 4.6×150
Quaternary ammonium 5 Styrene divinylbenzene copolymer 4.6×250
carboxyl 6 4.6×10
diol 7 2.0×150
Sulfo () 9 2.0×10
Sulfo () 10 10×150
Sulfo () 13 10×250
4.6×50
3.0×250
3.0×10
2.5×250
20×300
7.5×50
8×300
6×50
8×100
6×150
6×250

Manufacturers

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