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  11. Ion Exchange Chromatography (IEC)

Ion Exchange Chromatography (IEC)

  • Separation is based on electrostatic interactions between the ion exchanger and ionic solutes.
  • The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest charge differences between the analyte of interest.
  • The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase.
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Ion Exchange Chromatography (IEC)

Features
Specifications
Related products

Features

QA-825

DEAE-825
  1. Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA.
  2. Usable in a wide pH range from pH 2 to 12.
  3. QA-825 fulfills USP-NF L23 requirements.
ES-502N 7C
  1. Compared to IEC series column, polyvinyl alcohol is used as base material and this offers different separation pattern.
  2. Low hydrophobic interaction of proteins allows analysis under mild conditions.
SP-825

CM-825
  1. Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA.
  2. Usable in a wide pH range from pH 2 to 12

 
SP-FT 4A
  1. Non-porous base material.
  2. Provides ultra-rapid analysis conventional devices.
ES-502C 7C
  1. Compared to IEC series columns, polyvinyl alcohol is used as base material offering different separation pattern.
  2. Low hydrophobic interaction with protein allows analysis under mild conditions.
P-421S
  1. Column for amino acids analysis by cation exchange mode.
  2. Provides simultaneous analysis of different amino acids.
  3. Fulfills USP-NF L22 and L58 requirements.
YS-50
  1. High performance type of YK-421
  2. Applicable to both suppressor and non-suppressor methods
  3. Provides sharp peaks; more significant for divalent cation analysis
  4. Supports the analysis of alkylamines and transition metals
  5. Fulfills USP-NF L125 requirements

 
YK-421
  1. Column for cation analysis with non-suppressor method
  2. Simultaneous analysis of monovalent and divalent cations
  3. Suitable separating of alkylamines
  4. Fulfills USP-NF L76 requirements
NI-424

I-524A

 
  1. Ideal for anion non-suppressor methods
  2. NI-424 provides simultaneous analysis of fluoride and phosphate ions
  3. I-524A fulfills USP-NF L23 requirements

 
SI-90 4E

SI-50 4E

SI-52 4E

 
  1. Suitable for anion suppressor methods with sodium carbonate eluent
  2. Suitable for the quantitative analysis of fluoride ion
  3. SI-50 4E separates target inorganic anions from organic acids
  4. SI-52 4E provides simultaneous analysis of oxyhalides and general inorganic ions
  5. Carbonate peak does not interfere with analysis
SI-35
  1. Rapid-analysis type columns used with suppressor and sodium carbonate eluent
  2. SI-35 4D provides rapid analysis of oxyhalides and general inorganic ions
  3. SI-35 2B provides rapid analysis of general inorganic ions
SI-36 4D

SI-37 4D
  1. Suitable for anion suppressor methods with potassium hydroxide eluent
  2. SI-36 4D provides good separation of sulfite and sulfate ions
  3. SI-37 4D provides high sensitive analysis of oxyhalides in drinking water

Specifications

Functional group Particle size (µm) Base material Column size (mm)
IEC carboxyl 2.7 Polyvinyl alcohol 8×75
  Quaternary ammonium 3.5 Styrene divinylbenzene copolymer 7.5×100
  5 polyhydroxymethacrylate 4.6×10
  6 silica 4.6×150
  8 4.6×125
  9 4.6×100
  12 4×250
  4×150
  2×50

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