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PRODUCT

Size Exclusion Chromatography (SEC)

  • Network or pores on the surface of the packing material works as molecular sieve to separate molecules based on their sizes.
  • To separate molecules solely based on their sizes, it requires an analytical condition without any compounds and packing gel interaction.
  • The bigger the molecule size, the faster the elution sequence.
  • Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers.
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Size Exclusion Chromatography (SEC)

Features

KW-800

  1. Silica-based packed columns for aqueous SEC analysis.
  2. Suitable for the analysis of proteins and enzymes.
  3. Fulfills USP-NF L20, L33 and L59 requirements.
KW400
  1. Reduced packing material particle size enhances performance.
  2. Three to four-fold higher sensitivity than KW-800 series
  3. KW405-4F is applicable analyzing samples with molecular weight above 1000000
  4. Fulfills USP-NF L20, L33 and L59 requirements.
LW-803
  1. Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand.
  2. High performance analysis of antibody drugs and various proteins.
  3. High lot-to-lot reproducibility.
  4. Fulfills USP-NF L20, L33 and L59 requirements.
LW-403 4D
  1. Rapid analysis column of LW-803.
  2. Achieves approximately halved analysis time compared with standard column.
  3. Fulfills USP-NF L20, L33 and L59 requirements.
SB-800 HQ
  1. Polymer-based packed columns for aqueous SEC (GFC) analysis.
  2. Supports a wide range of molecular weight sample analysis.
  3. The eluent can be replaced with DMF (except SB-802 HQ for gelatin’s mean molecular weight determination is comparable with PAGI method (Ver. 10, Japan)
  4. Fulfills USP-NF, L38 and L39 requirements.
  5. SB-802 HQ fulfills USP-NF L25 requirements.
  6. SB-802.5 HQ fulfills USP-NF L25 requirements.
  7. SB-803 HQ fulfills USP-NF L37 requirements.
SB-807 HQ
  1. Column bleeding allows its use with light scattering detectors.
  2. The eluent can be replaced with DMF enabling the analysis of polar polymers.
  3. Fulfills USP-NF, L38 and L39 requirements.
LB-800
  1. Polymer-based packed columns for aqueous SEC (GFC) analysis.
  2. Low column bleeding allows its use with light scattering detectors.
  3. The eluent can be replaced with DMF enabling the analysis of polar polymers.
  4. Fulfills USP-NF, L38 and L39 requirements.
  5. LB-802.5 fulfills USP-NF L25 and L89 requirements.
  6. LB-803 fulfills USP-NF L37 requirements.
KF-800
  1. Standard organic solvent SEC (GPC) column.
  2. Supports a wide range of application from low to high molecular weight compounds.
  3. Fulfills USP-NF L21 requirements.
KD-800
  1. Standard organic solvent SEC (GPC) column.
  2. Supports a wide range of application from low to high molecular weight compounds.
  3. Fulfills USP-NF L21 requirements.
KF-400HQ
  1. About 1.5 times better separation performance than standard columns, obtain higher resolution.
  2. About 4 times better sensitivity than that of standard columns, supports high sensitivity analysis. The amount of solvent used is reduced to about a third.
  3. Improved applicability of solvent replacement.
  4. Fulfills USP-NF L21 requirements.
HK-400
  1. Newly developed styrene divinylbenzene copolymer monodisperse particles.
  2. Analysis time is reduced to about a sixth of conventional column’s analysis time.
  3. Low column pressure even under high flow rate does not require a UHPLC system.
  4. The amount of solvent used is reduced to about a sixth.
  5. Fulfills USP-NF L21 requirements.
LF
  1. Packed with unique multi-pore gels with a wide pore-size distribution.
  2. Highly linear calibration curve without inflection points.
  3. Achieves highly precise molecular weight distribution determination.
  4. Enables analysis over a wide molecular weight range.
  5. Rapid analysis column (LF-604) and high-performance analysis column (LF-404) are also available.
  6. LF-604 and LF-404 reduce solvent use.
  7. Fulfills USP-NF L21 requirements.
FP-2002
  1. Newly developed styrene divinylbenzene copolymer monodisperse particles.
  2. Can deliver t four times higher flow rate (10 ml/min or more) compared with conventional products.
  3. Achieves rapid recycling separation.
  4. Suitable for the separation of samples in a wide molecular weight range due to its wide linear range pore volume.
  5. Usable with various organic solvents such as THF, toluene, dichloroethane, ethyl acetate, DMF, and acetone used in GPC analysis in addition to chloroform.
SH1011, SH1821
  1. Columns for simultaneous analysis of saccharides and organic acids (counter ion: H+)
  2. Separates neutral sugars by size exclusion mode and organic acids by ion exclusion mode
  3. Suitable for the analysis of uronic and aldonic acids
  4. Fulfill USP-NF L17 and L22 requirements
SC1011

SP0810

KS-801

KS-802

 

  1. Separates saccharides by combination of ligand exchange and size exclusion modes
  2. Three types of counter ions are available: Ca2+, Pb2+ and Na+
  3. Only water is required for the analysis of neutral sugars
  4. SC1011 fulfills USP-NF L19 and L22 requirements
  5. SP0810 fulfills USP-NF L22 and L34 requirements
  6. KS-801 and KS-802 fulfill USP-NF L22 and L58 requirements
KF-800D
  1. Use this column in combination with a linear column
  2. Accurate molecular weight distribution of polymers and oligomers are achieved by shifting the elutions of monomers, polymer additives, and solvent-peak in the lower molecular region.

Specifications

Functional group Particle size (µm) Base material Column size (mm)
SEC sulfo 3 silica 8×300
  sulfo 3.5 Styrene divinylbenzene copolymer 6×50
  sulfo 5 4.6×300
  sulfo 6 4.6×10
  6.5 4.6×150
  7 4.6×20
  8 20×300
  10 8×50
  13 8×100
  15 4.6×250
  20 20×600
  35 6×250

Manufacturers

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Size Exclusion Chromatography

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