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  11. Reversed Phase Chromatography (RPC)

Reversed Phase Chromatography (RPC)

  • Separation is based on the partition equilibrium between stationary phase.
  • The polarity of the stationary phase is lower than that of the mobile phase.
  • Typically, the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
  • Use of lower polarity mobile phases fasten the elution.
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Reversed Phase Chromatography (RPC)

Features
Specifications
Document
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Features

ODP2 HP
  1. Provides a large theoretical plate number nearly twice as much as generally available polymer based reversed phase columns do.
  2. Offers enhanced retention of high polar substances compared to ODS columns.
  3. Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix.
  4. Ideal for LC/MS analysis of high polar compounds
  5. Fulfills USP_NF L39 requirements.
ODP-50 series, C4P-50 4D
  1. Relatively large pore size is suitable for analysis of amino acids, peptides, and proteins.
  2. Usable in a wide pH range from pH 2 to 13
  3. Usable in 100% water and buffer solution.
  4. Best used for the analysis of basic substances.
  5. ODP-50 fulfills USP-NF L67 requirements.
DS-413, DS-416, DM-416
DS-613

DS-416
  1. Suitable for reversed phases analysis of highly hydrophilic substances that are not well retained by ODS columns.
  2. Fulfill USP-NF L21 requirements.
DM-416
  1. Suitable in amino acids and water-soluble vitamins.
  2. Fulfill USP-NF L39 requirements.
DE series
DE-613

DE-416

DE-213
  1. General purpose polymer-based column having similar polarity as OSD columns.
  2. Wide working pH range (from pH 2 to 12), Usable in 100% water and buffer solution.
  3. Fulfill USP-NF L71 requirements.
SB-802.5 HQ, LB-802.5
SB-800 HQ
  1. Polymer-based packed columns for aqueous SEC (GFC) analysis.
  2. Supports a wide range of molecular weight sample analysis.
  3. The eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers.
  4. Method using SB-804 HQ or SB-805 HQ for gelatin’s mean molecular weight determination is comparable with PAGI method (Ver. 10, Japan)
  5. Fulfills USP-NF L38 and L39 requirements.
  6. SB-802 HQ fulfills USP-NF L25 requirements.
  7. SB-802.5 HQ fulfills USP-NF L25 and L89 requirements.
  8. SB-802 HQ fulfills USP-NF L37 requirements.
SB-807 HQ
  1. Column for the analysis of water-soluble ultra-high molecular weight polymers.
  2. Large particle-size gel prevents shear degradation of polymers.
  3. Fulfills USP-NF L39 requirements.
LB-800
  1. Polymer-based packed columns for aqueous SEC (GFC) analysis.
  2. Low column bleeding allows its use with light scattering detectors.
  3. The eluent can be replaced with DMF enabling the analysis of polar polymers.
  4. Fulfills USP-NF L38 and L39 requirements.
  5. LB-802.5 HQ fulfills USP-NF L25 requirements.
  6. LB-803 HQ fulfills USP-NF L37 requirements.
JJ-50 2D
  1. The packing material modified with trace amounts of quaternary ammonium groups, and supports multimode (reversed Phase and anion exchange) analysis.
  2. Ideal for the analysis of complex samples containing neutral and iconic substances.
C18M, C18U
C18M
  1. Monomeric type ODS column, fully end capped high purity silica (99.99% or higher)
  2. Fulfills USP-NF L1 requirements.
C18U
  1. ODS columns for UHPLC (Maximum pressure: 100 MPa).
  2. Achieves high performance analysis with sub-2 µm particles.
  3. Organic/inorganic silica hybrid particles provide excellent resolution and mechanical stability and improved alkali durability (from pH 1 to 12).
  4. Usable in 100% water and buffer solution.
  5. Fulfills USP-NF L1 requirements.
SH1011, SH1821
  1. Columns for simultaneous analysis of saccharides and organic acids (counter ion: H+)
  2. Separates neutral sugars by size exclusion mode and organic acids by ion exclusion mode
  3. Suitable for the analysis of uronic and aldonic acids
  4. Fulfill USP-NF L17 and L22 requirements
KC-811
  1. Columns suitable for the analysis of organic acids
  2. Separates compounds by ion exclusion mode and reversed phase mode
  3. Highly selective when used with post column method
  4. KC-811 6E is suitable for cyanide ions and cyanogen chloride analysis in accordance with the Japanese Water Supply Act
  5. Fulfills USP-NF L17 and L22 requirements

 
NN-814
  1. The packing material modified with sulfo groups supports multimode (reversed Phase and cation exchange) analysis.
  2. Ideal for the analysis of complex samples containing neutral and ionic substances.

Specifications

  Functional group Particle size (µm) Base material Column size (mm)
RPC octadecyl 1.9 polyhydroxymethacrylate 4.6×50
  sulfo 4 Polyvinyl alcohol 4.6×150
  Quaternary ammonium 5 polymethacrylate 4.6×250
  6 silica 2×50
  8 Organic/inorganic hybrid silica 2×150
  9 Styrene divinylbenzene copolymer 2×10
  10 10×250
  12 7.5×50
  13 6×150
  15 6×250
  18 6×10
  20 4.6×10
  35 20×300
  6×50
  8×250
  8×300
  8×50

Manufacturers

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Document

Reversed Phase Chromatography Brochure

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